Using benthic foraminifera to reconstruct the benthic environment during sapropel formation

Date du colloque : 03/2008International audienc

The role of a disulfide bridge in the stability and folding kinetics of Arabidopsis thaliana cytochrome c6A

Cytochrome c 6A is a eukaryotic member of the Class I cytochrome c family possessing a high structural homology with photosynthetic cytochrome c 6 from cyanobacteria, but structurally and functionally distinct through the presence of a disulfide bond and a heme mid-point redox potential of + 71 mV (vs normal hydrogen electrode). The disulfide bond is part of a loop insertion peptide that forms a cap-like structure on top of the core α-helical fold. We have investigated the contribution of the disulfide bond to thermodynamic stability and (un)folding kinetics in cytochrome c 6A from Arabidopsis thaliana by making comparison with a photosynthetic cytochrome c 6 from Phormidium laminosum and through a mutant in which the Cys residues have been replaced with Ser residues (C67/73S). We find that the disulfide bond makes a significant contribution to overall stability in both the ferric and ferrous heme states. Both cytochromes c 6A and c 6 fold rapidly at neutral pH through an on-pathway intermediate. The unfolding rate for the C67/73S variant is significantly increased indicating that the formation of this region occurs late in the folding pathway. We conclude that the disulfide bridge in cytochrome c 6A acts as a conformational restraint in both the folding intermediate and native state of the protein and that it likely serves a structural rather than a previously proposed catalytic role. © 2011 Elsevier B.V. All rights reserved

A Millimeter-wave Galactic Plane Survey with the BICEP Polarimeter

In order to study inflationary cosmology and the Milky Way Galaxy's composition and magnetic field structure, Stokes I, Q, and U maps of the Galactic plane covering the Galactic longitude range 260° < ℓ < 340° in three atmospheric transmission windows centered on 100, 150, and 220 GHz are presented. The maps sample an optical depth 1 ≾ AV ≾ 30, and are consistent with previous characterizations of the Galactic millimeter-wave frequency spectrum and the large-scale magnetic field structure permeating the interstellar medium. The polarization angles in all three bands are generally perpendicular to those measured by starlight polarimetry as expected and show changes in the structure of the Galactic magnetic field on the scale of 60°. The frequency spectrum of degree-scale Galactic emission is plotted between 23 and 220 GHz (including WMAP data) and is fit to a two-component (synchrotron and dust) model showing that the higher frequency BICEP data are necessary to tightly constrain the amplitude and spectral index of Galactic dust. Polarized emission is detected over the entire region within two degrees of the Galactic plane, indicating the large-scale magnetic field is oriented parallel to the plane of the Galaxy. A trend of decreasing polarization fraction with increasing total intensity is observed, ruling out the simplest model of a constant Galactic magnetic field orientation along the line of sight in the Galactic plane. A generally increasing trend of polarization fraction with electromagnetic frequency is found, varying from 0.5%-1.5% at frequencies below 50 GHz to 2.5%-3.5% above 90 GHz. The effort to extend the capabilities of BICEP by installing 220 GHz band hardware is described along with analysis of the new band

Recent results from the CMB

We review recent strides in understanding the universe through measurements of anisotropies in the cosmic microwave background radiation. Particular emphasis is given to the BOOMERanG experiment and results

Models of dust around Europa and Ganymede

We use numerical models, supported by our laboratory data, to predict the dust densities of ejecta outflux at any altitude within the Hill spheres of Europa and Ganymede. The ejecta are created by micrometeoroid bombardment and five different dust populations are investigated as sources of dust around the moons. The impacting dust flux (influx) causes the ejection of a certain amount of surface material (outflux). The outflux populates the space around the moons, where a part of the ejecta escapes and the rest falls back to the surface. These models were validated against existing Galileo DDS (Dust Detector System) data collected during Europa and Ganymede flybys. Uncertainties of the input parameters and their effects on the model outcome are also included. The results of this model are important for future missions to Europa and Ganymede, such as JUICE (JUpiter ICy moon Explorer), recently selected as ESA's next large space mission to be launched in 2022

Radial Velocity Studies of Close Binary Stars. XV

Radial-velocity measurements and sine-curve fits to the orbital radial velocity variations are presented for the last eight close binary systems analyzed the same way as in the previous papers of this series: QX And, DY Cet, MR Del, HI Dra, DD Mon, V868 Mon, ER Ori, and Y Sex. For another seven systems (TT Cet, AA Cet, CW Lyn, V563 Lyr, CW Sge, LV Vir and MW Vir) phase coverage is insufficient to provide reliable orbits but radial velocities of individual components were measured. Observations of a few complicated systems observed throughout the DDO close-binary program are also presented; among them an especially interesting is the multiple system V857 Her which - in addition to the contact binary - very probably contains one or more sub-dwarf components of much earlier spectral type. All suspected binaries which were found to be most probably pulsating stars are briefly discussed in terms of mean radial velocities and projected rotation velocities (v sin i) as well as spectral type estimates. In two of them, CU CVn and V752 Mon, the broadening functions show a clear presence of non-radial pulsations. The previously missing spectral types for the DDO I paper are given here in addition to such estimates for most of the program stars of this paper.Comment: submitted to A

Mapping the CMB Sky: The BOOMERANG experiment

We describe the BOOMERanG experiment, a stratospheric balloon telescope intended to measure the Cosmic Microwave Background anisotropy at angular scales between a few degrees and ten arcminutes. The experiment has been optimized for a long duration (7 to 14 days) flight circumnavigating Antarctica at the end of 1998. A test flight was performed on Aug.30, 1997 in Texas. The level of performance achieved in the test flight was satisfactory and compatible with the requirements for the long duration flight.Comment: 11 pages, 6 figure

CMB polarimetry with BICEP: instrument characterization, calibration, and performance

BICEP is a ground-based millimeter-wave bolometric array designed to target the primordial gravity wave signature on the polarization of the cosmic microwave background (CMB) at degree angular scales. Currently in its third year of operation at the South Pole, BICEP is measuring the CMB polarization with unprecedented sensitivity at 100 and 150 GHz in the cleanest available 2% of the sky, as well as deriving independent constraints on the diffuse polarized foregrounds with select observations on and off the Galactic plane. Instrument calibrations are discussed in the context of rigorous control of systematic errors, and the performance during the first two years of the experiment is reviewed.Comment: 12 pages, 15 figures, updated version of a paper accepted for Millimeter and Submillimeter Detectors and Instrumentation for Astronomy IV, Proceedings of SPIE, 7020, 200

SPIDER: a new balloon-borne experiment to measure CMB polarization on large angular scales

We describe SPIDER, a novel balloon-borne experiment designed to measure the polarization of the Cosmic Microwave Background (CMB) on large angular scales. The primary goal of SPIDER is to detect the faint signature of inflationary gravitational waves in the CMB polarization. The payload consists of six telescopes, each operating in a single frequency band and cooled to 4 K by a common LN/LHe cryostat. The primary optic for each telescope is a 25 cm diameter lens cooled to 4 K. Each telescope feeds an array of antenna coupled, polarization sensitive sub-Kelvin bolometers that covers a 20 degree diameter FOV with diffraction limited resolution. The six focal planes span 70 to 300 GHz in a manner optimized to separate polarized galactic emission from CMB polarization, and together contain over 2300 detectors. Polarization modulation is achieved by rotating a cryogenic half-wave plate in front of the primary optic of each telescope. The cryogenic system is designed for 30 days of operation. Observations will be conducted during the night portions of a mid-latitude, long duration balloon flight which will circumnavigate the globe from Australia. By spinning the payload at 1 rpm with the six telescopes fixed in elevation, SPIDER will map approximately half of the sky at each frequency on each night of the flight

The Robinson Gravitational Wave Background Telescope (BICEP): a bolometric large angular scale CMB polarimeter

The Robinson Telescope (BICEP) is a ground-based millimeter-wave bolometric array designed to study the polarization of the cosmic microwave background radiation (CMB) and galactic foreground emission. Such measurements probe the energy scale of the inflationary epoch, tighten constraints on cosmological parameters, and verify our current understanding of CMB physics. Robinson consists of a 250-mm aperture refractive telescope that provides an instantaneous field-of-view of 17 degrees with angular resolution of 55 and 37 arcminutes at 100 GHz and 150 GHz, respectively. Forty-nine pair of polarization-sensitive bolometers are cooled to 250 mK using a 4He/3He/3He sorption fridge system, and coupled to incoming radiation via corrugated feed horns. The all-refractive optics is cooled to 4 K to minimize polarization systematics and instrument loading. The fully steerable 3-axis mount is capable of continuous boresight rotation or azimuth scanning at speeds up to 5 deg/s. Robinson has begun its first season of observation at the South Pole. Given the measured performance of the instrument along with the excellent observing environment, Robinson will measure the E-mode polarization with high sensitivity, and probe for the B-modes to unprecedented depths. In this paper we discuss aspects of the instrument design and their scientific motivations, scanning and operational strategies, and the results of initial testing and observations.Comment: 18 pages, 11 figures. To appear in Millimeter and Submillimeter Detectors and Instrumentation for Astronomy III, Proceedings of SPIE, 6275, 200